关键词: 红鲫, 鲤疱疹病毒2型, 聚维酮碘, 病毒阻断

Abstract: Objective　To investigate the effects of povidone-iodine immersion treatment on the embryonic development of red crucian carp (Carassius auratus), and explore its blocking effect on Cyprinid herpesvirus 2 (CyHV-2), providing a technical solution for viral eradication in experimental animal populations of red crucian carp.Methods　Clinically abnormal individuals were selected from a closed red crucian carp population. Viruses were isolated using cell culture method, and viral species were identified through PCR amplification and DNA sequencing. Gradient concentrations of povidone-iodine solution (10.0, 20.0, 30.0, 40.0 mg/mL) were applied for 5-minute immersion treatment on fertilized eggs. Safe and effective treatment protocols were screened by statistically analyzing embryonic hatching rates and malformation rates. Treated individuals at different developmental stages were sampled periodically, and CyHV-2 infections were tracked using PCR technology. Results　Viruses were successfully isolated from two diseased individuals in a closed population aquarium and confirmed as CyHV-2 through molecular biological identification. Experiments showed that povidone-iodine treatments at concentrations of 10.0, 20.0, 30.0, and 40.0 mg/mL for 5 minutes did not significantly affect embryonic development. No CyHV-2 virus was detected in any period of the group treated with 40.0 mg/ mL povidone-iodine for 5 minutes.Conclusion　Povidone-iodine treatment of red crucian carp fertilized eggs can block the transmission of CyHV-2 and could be developed into a standard method for viral eradication in experimental fish populations.

Key words: red crucian carp, cyprinid herpesvirus type 2, povidone-iodine, virus blocking